ESTD PERPUSTAKAAN UNTAD
ESTD PERPUSTAKAAN UNTAD
| JudulIdentifikasi Bakteri Escherichia Coli Dengan Pemeriksaan Kultur Dan Morfologi Di Taman Wisata Pemandian Air Panas Bora Sigi |
| Nama: FADHILAH SALSABILAH A GULI |
| Tahun: 2026 |
| Abstrak IDENTIFICATION OF Escherichia coli BACTERIA USING CULTURE AND MORPHOLOGICAL EXAMINATION AT BORA SIGI HOT SPRING TOURISM AREA Fadhilah Salsabilah A. Guli¹, M. Sabir², Ressy Dwiyanti³, Andi Nur Asrinawaty? ¹ Medical Study Program, Faculty of Medicine, Universitas Tadulako ² Department of Microbiology, Division of Tropical Infections and Traumatology, Faculty of Medicine, Universitas Tadulako ³ Department of Microbiology, Faculty of Medicine, Universitas Tadulako ? Department of Microbiology, Faculty of Medicine, Universitas Tadulako ABSTRACT Background: Natural hot spring water is often considered sterile due to its high temperature and mineral content, such as sulfur, which has antibacterial properties. However, human activities and environmental conditions may lead to microbiological contamination. Objective: This study aimed to identify the presence of Escherichia coli bacteria in the water of the Bora Hot Spring, Sigi Regency, using culture methods and morphological examination. Methods: This study employed a descriptive observational design. The study population consisted of microorganisms present in the hot spring pool water at Bora Hot Spring. Water samples were collected from three different pool points at three different time intervals. Bacterial identification was carried out through culture examination on nutrient agar using the pour plate method and microscopic observation using Gram staining as the morphological identification instrument. Results: The results showed that most samples were suspected to be positive for Escherichia coli, particularly in the first and third pools. The colonies observed were circular in shape with convex surfaces and smooth edges. Gram staining revealed Gram-negative, short rod-shaped bacteria. Of the total 30 isolates obtained, 7 isolates (23.33%) were suspected to be identified as Escherichia coli. Conclusion: The Bora Sigi Hot Spring was suspected to be contaminated with Escherichia coli bacteria. Keywords: Bora Sigi Hot Spring, Escherichia coli, bacterial culture, Gram |