ESTD PERPUSTAKAAN UNTAD
ESTD PERPUSTAKAAN UNTAD
| JudulIsolasi Dan Skrining Uji Toksisitas Isolat Jamur Endofit Yang Berasal Dari Tumbuhan Benalu Batu (Begonia Medicinalis) Menggunakan Metode Brine Shrimp Lethality Test (BSLT) |
| Nama: MUSYAHIDAH |
| Tahun: 2021 |
| Abstrak Endophytic fungi are microorganisms found in plant tissues that are mutually beneficial to the host plant. Benalu Batu (Begonia medicinalis) has been used empirically and shown anticancer activity on breast cancer cells (T47D cells) and cervical cancer cells (HeLa cells). This study aimed to determine the toxicity of endophytic fungal isolates against shrimp larvae (Artemia salina) and the Lethal Concentration 50% (LC50) value. Endophytic fungi isolates were extracted by sonication method using ethyl acetate as solvent, followed by the solvent evaporation process until a thick extract was obtained. The toxicity test of fungi isolates used the BSLT method with series concentrations of 500, 250, 125, 62.5, 31.25, 15.62, 7.8 and 3.9 ?g/mL. Isolation of the endophytic fungi of the Benalu Batu plant obtained 10 isolates, which the leaf part obtained 6 isolates, the stem obtained 3 isolates and the root obtained 1 isolate. The results of screening for toxicity test of 10 isolates at concentrations of 500 ug/mL, leaf isolate shown that D3, D5 and D6 gave 100% mortality. The LC50 values of D3, D5, and D6 were obtained respectively at 448.03, 8.784 and 9.13 ?g/mL. These results indicated that D5 have greater toxicity and need to be investigated the activity for testing on cancer cells. |