ESTD PERPUSTAKAAN UNTAD
ESTD PERPUSTAKAAN UNTAD
| JudulIDENTIFIKASI DAN UJI KEMAMPUAN KOLONISASI MMIKORIZA ARBUSKULAR ASAL KAWASAN MANGROVE TAMAN NASIONAL RAWA AOPA SULAWESI TENGGARA |
| Nama: KOMANG PUTRA |
| Tahun: 2025 |
| Abstrak Komang Putra, Identification and Colonization Ability Test of Arbuscular Mycorrhizae from Mangrove Area in Rawa Aopa National Park, Southeast Sulawesi (Supervised by Nur Edy and Hasriyanty). Arbuscular mycorrhizal (AM) fungi, belonging to the Glomeromycetes division, form mutualistic endomycorrhizal associations and exhibit significant abundance and taxonomic diversity in terrestrial plant communities. Rawa Aopa Watumohai National Park (TNRAW) encompasses diverse vegetation types, including mangroves. Nevertheless, reports on mycorrhizal taxa abundance in this ecosystem remain limited. This study aimed to identify AM fungal abundance in TNRAW's mangrove area and evaluate colonization capacity through single-culture techniques using Bermuda grass (Cynodon dactylon) as the host plant. Identification employed the wet sieving method, while colonization assessment followed a Completely Randomized Design (CRD) with 3 treatments, 5 replications, and 3 controls (18 experimental pots). Six AM fungal genera wereiidentified: Gigaspora, Glomus, Acaulospora, Racocetra, Funneliformis, Den tiscuta, and Rhizophagus, with a total of 2,184 isolated spores. Diversity indices (S' and H') indicated dominance by Gigaspora and Glomus (>1.0 and >0.8, respectively). Single-culture root colonization revealed medium-to-high levels of vesicle and hyphal infections (25%–63%), with Glomus exhibiting the highest hyphal percentage. Successful spore propagation (>25 spores) was achieved in single cultures of Acaulospora. Quantitative host growth parameters (shoot height, leaf count, root length, and biomass) demonstrated significantly enhanced growth rates in treated plants versus controls. Optimal shoot height (24 cm) occurred in Glomus-inoculated C. dactylon, with statistically significant treatment effects (ANOVA-BNT LSD, p<0>6 cm versus control roots <3.6 cm. Keywords: Mycorrhizae, Colonization, Spores, Mangrove, Glomeromycetes. |