ESTD PERPUSTAKAAN UNTAD
ESTD PERPUSTAKAAN UNTAD
| JudulUji Kemampuan Bakteri Pelarut Fosfat Asal Rhizosfer Padi Sawah Intensif Di Kecamatan Gumbasa |
| Nama: FITRIANA |
| Tahun: 2024 |
| Abstrak Phosphate solubilizing bacteria are soil bacteria that are able to dissolve phosphate that is bound in the soil and released by converting the bound phosphate into decomposed phosphate so that it can be easily absorbed by plants. This research aims to identify phosphate solubilizing bacteria in the soil from the rice rhizosphere in intensive rice fields in Gumbasa District, Sigi Regency. This research was carried out from July to August 2023 at the Pharmaceutical Biotechnology Laboratory, Faculty of Agriculture, Tadulako University, Palu. This research used an exploratory descriptive method which was carried out by testing the solubility of phosphate solvent bacteria. The tools used in carrying out this research were petri dishes, test tubes, analytical scales, incubators, tissue, measuring cups, autoactives, heat-resistant plastic, aluminum foil, label paper, Ph meter, oven, graph paper, 10,000 rpm centrifuge, hot- plate, cotton, tube needle, sprayer, camera, mask, gloves, bunsen, microscope, object glass, object cover, fax, and spectrophotometer. The materials used for bacterial isolation were rice soil samples (taken from the Pakuli area, Gumbasa subdistrict), distilled water, liquid Pikovskaya media. Media composition per liter of distilled water: (10 g of glucose, 10 grams, put into 90 ml of 0.85% physiological gam solution, then incubated using a shaker for 3 hours, at room temperature with an agitation speed of 120 rpm. The research results are shown in the sample code S1(1) with a dissolution index of 50.28 Mg/l, sample code S1(2) 22.42 Mg/l, sample code S1(3) 24.42 Mg/l, sample code S2 101.9 Mg/l, sample code S3 89.4 Mg/l, sample code BS(1) 54.47 Mg/l, sample code BS(2) 54.38 Mg/l, sample code BS(3) 53.47 Mg/l, code sample BS(4) 50.95 Mg/l, sample code BS(5) 26.38 Mg/l. Sample codes S2 and S3 have the highest IPF values. Meanwhile sample codes S1(1), S1(2), S1(3). ),BS1, BS2, BS3, BS4, BS5, have the lowest IPF values. There are 2 types of phosphate-dissolving bacteria, namely Bacillus Megaterium and Bacillus sphaericus. The aim of this research is to identify rice rhizosphere bacteria that have the ability to dissolve phosphate used came from Pakuli Village, Gumbasa District, Sigi Regency, the method used in taking soil samples was polar sampling, where at each location 3 soil sample points were taken at random and at each soil sample point 3 polar samples were taken. Soil samples were taken at a distance of 30 cm from the base of the roots with a depth of 5 to 20 cm around the plant roots. The soil was then composited and put into a plastic bag and then taken to the laboratory (Saraswati et al., 2012). |